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igg1 apc nkp30 miltenyi biotec  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec igg1 apc nkp30 miltenyi biotec
    Igg1 Apc Nkp30 Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd314+apc/CD314+(NKG2D)+Antibody%2C+anti-human%2C+APC%2C+REAfinity/pmc11386223__szae047_suppl_supplementary_table_s3-2-190-192
    Average 93 stars, based on 6 article reviews
    igg1 apc nkp30 miltenyi biotec - by Bioz Stars, 2026-09
    93/100 stars

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    Bioprocessing:

    Article Title: Cytokine-Induced Killer Cells Express CD39, CD38, CD203a, CD73 Ectoenzymes and P1 Adenosinergic Receptors
    Article Snippet: .. The following monoclonal antibodies (mAb) were used: CD3-FITC, CD4-PE, CD56-APC, CD8-PE, and CD314-APC (anti-NKG2D) (all mAb are from Miltenyi Biotec S.r.l., BO, Italy). .. Labeled cells were read on FACS Cyan (Cyan ADP, Beckman Coulter s.r.l., Cassina De' Pecchi, MI, Italy) and analyzed using Summit Software.

    Article Title: Preclinical immunotherapy with Cytokine-Induced Killer lymphocytes against epithelial ovarian cancer
    Article Snippet: .. The following monoclonal antibodies (mAb) were used: CD3 (Anti-Human CD3 FITC Mouse, BD PHarmingenTM), CD4 (Anti-Human CD4 PE, MACS MiltenyiBiotec), CD8 (Anti-Human CD8 PE, MACS MiltenyiBiotec), CD56 (Anti-Human CD56 APC, MACS MiltenyiBiotec) and CD314–APC (anti-NKG2D; MACS MiltenyiBiotec). ..

    Article Title: Analytic and Dynamic Secretory Profile of Patient-Derived Cytokine-Induced Killer Cells
    Article Snippet: .. The following monoclonal antibodies (mAbs) were used: CD3-FITC, CD4 – PE, CD56-APC, CD8 – PE and CD314-APC (anti-NKG2D) (all mAbs were from Miltenyi Biotec). .. The Miltenyi Biotec Treg Detection kit (CD4/CD25/Foxp3) (APC) was used to detect T regulatory cells within CIK cultures at d 14 and 21.

    Article Title: Cytokine-induced killer cells efficiently kill stem-like cancer cells of nasopharyngeal carcinoma via the NKG2D-ligands recognition.
    Article Snippet: Phenotypes of CIK cells were weekly analyzed with a FACSCalibur flow cytometry (BD Biosciences). .. The following monoclonal antibodies (mAb) were used: CD3-FITC, CD4-FITC, CD8-PE, CD56-APC and CD314-APC (anti-NKG2D) (Miltenyi, Germany). ..

    Article Title: Cytokine-induced killer cells efficiently kill stem-like cancer cells of nasopharyngeal carcinoma via the NKG2D-ligands recognition
    Article Snippet: Phenotypes of CIK cells were weekly analyzed with a FACSCalibur flow cytometry (BD Biosciences). .. The following monoclonal antibodies (mAb) were used: CD3-FITC, CD4-FITC, CD8-PE, CD56-APC and CD314-APC (anti-NKG2D) (Miltenyi, Germany). ..

    Article Title: Recognition and killing of cancer stem-like cell population in hepatocellular carcinoma cells by cytokine-induced killer cells via NKG2d-ligands recognition
    Article Snippet: .. Cells were obtained from CIK cell cultures for phenotype analysis with the appropriated monoclonal antibodies (mAb), including CD3-FITC, CD4-FITC, CD8-PE, CD56-APC, and CD314–APC (anti-NKG2D) (Miltenyi, Germany) by standard flow cytometric assays (BD Biosciences). ..

    Article Title: Cytokine-Induced Killer Cells Kill Chemo-surviving Melanoma Cancer Stem Cells
    Article Snippet: Briefly, PBMCs, isolated by density gradient (Lymphosep, Aurogene s.r.l., Roma, Italy) centrifugation were cultured at a cell density of 1.5 × 106 ml−1 in RPMI (Gibco BRL Life Technologies Italia Monza Italy), supplemented with 10% fetal bovine serum (FBS) (Sigma) and with timed additions of 1000 U ml−1 IFN-γ at day 0 (Miltenyi Biotec Srl, 6 Calderara di Reno, BO, Italy), 50 ng ml−1 anti-CD3 at day+1 antibody (Miltenyi Biotec Srl, Calderara di Reno, BO, Italy), and 300 U ml−1 recombinant human interleukin IL-2 (from day +1, refreshed every 3-4 days until the end of the expansion) (Miltenyi Biotec Srl, Calderara di Reno, BO, Italy). .. Phenotypic analysis of CIK cells was performed weekly, using the following fluorescein isothiocyanate (FITC), phycoerythrin (PE), or allophycocyanin (APC)-conjugated mouse monoclonal antibodies (mAbs): CD3–FITC, CD8–PE, CD56–APC and CD314–APC (aka anti-NKG2D) (mAbs) (all from Miltenyi Biotec Srl, Calderara di Reno, BO, Italy) and anti-DNAM-1 (Becton Dickinson BD Biosciences Italy, Pharmingen). .. Labeled cells were read on FACS Cyan (Cyan ADP, Beckman Coulter s.r.l., Cassina De' Pecchi – Milan, Italy) and analyzed using Summit Software.

    Magnetic Cell Separation:

    Article Title: Preclinical immunotherapy with Cytokine-Induced Killer lymphocytes against epithelial ovarian cancer
    Article Snippet: .. The following monoclonal antibodies (mAb) were used: CD3 (Anti-Human CD3 FITC Mouse, BD PHarmingenTM), CD4 (Anti-Human CD4 PE, MACS MiltenyiBiotec), CD8 (Anti-Human CD8 PE, MACS MiltenyiBiotec), CD56 (Anti-Human CD56 APC, MACS MiltenyiBiotec) and CD314–APC (anti-NKG2D; MACS MiltenyiBiotec). ..



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    Image Search Results


    Representative dot plots showing the CD314 (NKG2D), CD28, CD279 (PD-1), granzyme-B and perforin expression of CD8+CD20+ T-cells.

    Journal: Frontiers in Immunology

    Article Title: Multiparameter flow cytometric and transcriptional analyis of CD20 positive T-cells in bone marrow in patients of multiple myeloma and monoclonal gammopathy of undetermined significance

    doi: 10.3389/fimmu.2025.1464940

    Figure Lengend Snippet: Representative dot plots showing the CD314 (NKG2D), CD28, CD279 (PD-1), granzyme-B and perforin expression of CD8+CD20+ T-cells.

    Article Snippet: Fluorescent dyes and manufacturers of the additional monoclonal antibodies for phenotyping are briefly: BB515-conjugated anti-CD279 (clone: EH12.1, BD Biosciences), PE-Cy7-conjugated anti CD28 (clone: CD28.2, Sony Biotechnology), APC-conjugated anti-CD314 or NKG2D (clone: BcR-A-CT35, Sony Biotechnology), BV510-conjugated anti-CD8 (clone: SK1, BD-Biosciences), FITC-conjugated Granzyme-B (clone: Gb 11, Beckman-Coulter), APC-conjugated anti-CD25 (clone: M-A251, Sony Biotechnology), APC-R700 anti-CD223 (clone: T47-530, BD Biosciences), APC-conjugated anti CD137 (clone: 4B4-1, Sony Biotechnology), FITC-conjugated Perforin (clone: delta G9, Miltenyi Biotec).

    Techniques: Expressing